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These two figures represent the rice kinase interaction maps. They are composed of data generated from high throughput yeast two hybrid, and in vivo nTAP affinity pulldowns screens using rice kinases. We used Cytoscape software to generate maps of the nTAP and Y2H interacting protein pairs. Kinases used as "baits" are squares, and colored yellow. The interactors identified by these baits are represented as blue circles. Clicking on a node (protein) will link to the TIGR data base so that viewers can see detailed information on each component in the complex. Detailed analysis of each of the kinase baits and its interactors can be accessed using the tree viewer and selecting the Yeast Two-hybrid Bait or Tandem Affinity Purification-tagged Bait icon. This will show Locus IDs of kinases that have interactors. Clicking on the Locus ID will show you the interactors and annotation.

* This page can work properly on Mozilla Firefox and Opera, but for Windows Internet Explorer and Macintosh Safari, the Adobe SVG viewer plug-in is required for viewing the map and can be freely downloaded.
* You can zoom in/out by right click on the interaction map.

Last modified: Saturday, 03-Jul-2010 19:37:36 PDT

Yeast Two Hybrid Map

Ding X, Richter T, Chen M, Fujii H, Seo YS, Xie M, Zheng X, Kanrar S, Stevenson RA, Dardick C, Li Y, Jiang H, Zhang Y, Yu F, Bartley LE, Chern M, Bart R, Chen X, Zhu L, Farmerie WG, Gribskov M, Zhu JK, Fromm ME, Ronald PC, Song WY. 2009. A rice kinase-protein interaction map. Plant Physiol. 149(3):1478-92.

TAPtag Map

1) Rohila JS, Chen M, Chen S, Chen J, Cerny R, Dardick C, Canlas P, Xu X, Gribskov M, Kanrar S, Zhu J-K, Ronald P and Fromm ME. 2006. Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice. The Plant Journal. 46, 1-13.

2) Rohila JS, Chen M, Chen S, Chen J, Cerny R, Dardick C, Canlas P, Fuji H, Gribskov M, Kanrar S, Knoflicek L. Stevenson B, Xie M, Xu X,Zheng X, Zhu J-K, Ronald P and Fromm ME. 2008. Protein-Protein Interactions of TAP-Tagged Protein Kinases in Rice. Under Revision for Molecular and Cellular Biology.

UC Davis Zhejiang University Indica RDA SSAC Last Update: June 2015